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Anti-CGI-58 antibody antibody detects adipose triglyceride lipase protein. CGI-58 is required for the hydrolysis of fatty acids to generate energy and prevents cardiomyocyte lipid accumulation and dysfunction. Inhibition of triglyceride lipase increases intracellular lipid accumulation, impairs mitochondrial oxidative phosphorylation, and induces hypertrophic cardiac remodeling in mice. Cardiac-specific overexpression of CGI-58 reduces lipid accumulation and alleviates pressure overload-induced heart failure (HF) in mice.

The CGI-58 gene encodes a lysophosphatidic acid acyltransferase that preferentially accepts arachidonoyl-CoA and oleoyl-CoA as acyl donors. Mutations in CGI-58 result in Dorfman-Chanarin syndrome, a neutral lipid storage disorder with apparent ichthyosis. CGI-58 has been shown to act as an ATGL activator and promote lipid hydrolysis. However, its acyltransferase activity toward other lysophospholipids has been unknown. To address this, we generated a FLAG-tagged full-length CGI-58 cDNA and expressed it in insect cells. The recombinant CGI-58 protein exhibited LPAAT activity toward phosphatidic acid (PG) and lysophosphatidylcholine (LPC), but not lipid peroxidation products or other lysophospholipids, as determined by LC-MS analysis.

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Immunoblotting and immunoelectron microscopy confirmed that CGI-58 is localized to the lamellar granules (LGs), a group of lipid transport and secretion vesicles found in keratinocytes. In addition, LG-specific deletion of CGI-58 results in reduced PG and CL content in cultured skin fibroblasts and Dorfman-Chanarin syndrome patients. The restoration of CGI-58 expression by adeno-associated virus-9-mediated gene transfer (rAAV9-CGI-58) alleviated lipid accumulation and ER stress in CGI-58f/f and CGI-58cko mice fed a normal or HFD diet. 4-PBA treatment further mitigated lipid accumulation and ER stress in both CGI-58f/f and CGI-58cko hearts.

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Top-rated police misconduct lawyer in Seattle can be a terrifying experience for anyone who is the victim of it. Depending on the severity of the case, victims may choose to report it as an internal police matter by filing a complaint with their local department or file a civil lawsuit against the officer involved for damages. In 2010 alone, millions of dollars in civil settlements and judgments were awarded to the victims of police misconduct. The main types of police misconduct include harassment, police brutality, false imprisonment and wrongful death.

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Losing a loved one brings with it a host of emotional and logistical responsibilities. Among these are notifying family members, clearing out property, and filing paperwork with the courts. But perhaps the most stressful responsibility is estate administration. This involves the proper distribution of a deceased person’s assets—and in New York, this requires probate. While settling an estate can be complicated, quality legal help can make the process less overwhelming. URL https://qldestatelawyers.com.au/probate-lawyers/

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